Use el DOI o este identificador para enlazar este recurso: http://ru.facmed.unam.mx/jspui/handle/FACMED_UNAM/A12
Título : Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix.
Autor(es) : Rodriguez Fuentes, Nayeli
Reynoso Ducoing, Olivia Alicia
Rodríguez, Ana
Ambrosio Hernandez, Javier Rolando
Piña Barba, Maria Cristina
Zepeda, Armando
Cerbon Cervantes, Marco Antonio
Tapia Ramírez, Jose Isabel
Alcantara Quintana, Luz Eugenia
En : Journal of Visualized Experiments (1940-087X ) vol. 9(96), 1-7 (2015)
Número completo : https://www.jove.com/archive/96/february-2015#biology
Abstract : Mesenchymal stem cells (MSCs) have a differentiation potential towards osteoblastic lineage when they are stimulated with soluble factors or specific biomaterials. This work presents a novel option for the delivery of MSCs from human amniotic membrane (AM-hMSCs) that employs bovine bone matrix Nukbone (NKB) as a scaffold. Thus, the application of MSCs in repair and tissue regeneration processes depends principally on the efficient implementation of the techniques for placing these cells in a host tissue. For this reason, the design of biomaterials and cellular scaffolds has gained importance in recent years because the topographical characteristics of the selected scaffold must ensure adhesion, proliferation and differentiation into the desired cell lineage in the microenvironment of the injured tissue. This option for the delivery of MSCs from human amniotic membrane (AM-hMSCs) employs bovine bone matrix as a cellular scaffold and is an efficient culture technique because the cells respond to the topographic characteristics of the bovine bone matrix Nukbone (NKB), i.e., spreading on the surface, macroporous covering and colonizing the depth of the biomaterial, after the cell isolation process. We present the procedure for isolating and culturing MSCs on a bovine matrix.
Palabras clave : Biología del desarrollo
Células troncales mesenquimales humanas
Biomateriales porosos
Ingenieria de tejido óseo
Amnios
Fecha de publicación : 2015
DOI : 10.3791/51999
URI : http://ru.facmed.unam.mx/jspui/handle/FACMED_UNAM/A12
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