Use el DOI o este identificador para enlazar este recurso:
http://ru.facmed.unam.mx/jspui/handle/FACMED_UNAM/A107
Título : | Differential adhesion and fibrinolytic activity of mesenchymal stem cells from human bone marrow, placenta, and Wharton’s jelly cultured in a fibrin hydrogel. |
Autor(es) : | Chaires Rosas, Casandra Paulina Ambriz Peña, Xochitl Montesinos Montesinos, Juan Jose Hernandez Tellez, Beatriz Piñon Zarate, Gabriela Herrera Enriquez, Miguel Angel Hernandez Estevez, Erika Ayde Ambrosio Hernandez, Javier Rolando Castell Rodriguez, Andres Eliu |
En : | Journal of Tissue Engineering (2041-7314) Vol. 10, 1-17 (2019) |
Número completo : | https://journals.sagepub.com/toc/teja/10 |
Abstract : | Mesenchymal stem cells isolated from different tissues should share associated markers and the capability to differentiate to mesodermal lineages. However, their behavior varies in specific microenvironments. Herein, adhesion and fibrinolytic activity of mesenchymal stem cells from placenta, bone marrow, and Wharton’s jelly were evaluated in fibrin hydrogels prepared with nonpurified blood plasma and compared with two-dimensional cultures. Despite the source, mesenchymal stem cells adhered through focal adhesions positive for vinculin and integrin ?V in two dimensions, while focal adhesions could not be detected in fibrin hydrogels. Moreover, some cells could not spread and stay rounded. The proportions of elongated and round phenotypes varied, with placenta mesenchymal stem cells having the lowest percentage of elongated cells (~10%). Mesenchymal stem cells degraded fibrin at distinct rates, and placenta mesenchymal stem cells had the strongest fibrinolytic activity, which was achieved principally through the plasminogen–plasmin axis. These findings might have clinical implications in tissue engineering and wound healing therapy. |
Palabras clave : | Células troncales mesenquimales humanas Fibrina Adhesión Fibrinólisis |
Fecha de publicación : | 2019 |
DOI : | 10.1177/2041731419840622 |
URI : | http://ru.facmed.unam.mx/jspui/handle/FACMED_UNAM/A107 |
Aparece en las colecciones: | Artículos |
Texto completo:
Archivo | Descripción | Tamaño | Formato | |
---|---|---|---|---|
FACMED_UNAMA107.pdf | 2.21 MB | Adobe PDF | Visualizar/Abrir |
Este recurso está sujeto a una Licencia Creative Commons