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http://ru.facmed.unam.mx/jspui/handle/FACMED_UNAM/A64
Título : | 2D-immunoblotting analysis of Sporothrix schenckii cell wall. |
Autor(es) : | Ruiz Baca, Estela Mora Montes, Hector Manuel Lopez Romero, Everardo Toriello Najera, Concepcion Mojica Marin, Virgilio Urtiz Estrada, Norma |
En : | Memórias do Instituto Oswaldo Cruz (0074-0276) vol. 106(2), 248-50 (2011) |
Número completo : | https://memorias.ioc.fiocruz.br/content/past-issues/past/16 |
Abstract : | We utilized two-dimensional gel electrophoresis and immunoblotting (2D-immunoblotting) with anti-Sporothrix schenckii antibodies to identify antigenic proteins in cell wall preparations obtained from the mycelial and yeast-like morphologies of the fungus. Results showed that a 70-kDa glycoprotein (Gp70) was the major antigen detected in the cell wall of both morphologies and that a 60-kDa glycoprotein was present only in yeast-like cells. In addition to the Gp70, the wall from filament cells showed four proteins with molecular weights of 48, 55, 66 and 67 kDa, some of which exhibited several isoforms. To our knowledge, this is the first 2D-immunoblotting analysis of the S. schenckii cell wall. |
Palabras clave : | Micología Sporothrix schenckii Pared celular Glicoproteínas |
Fecha de publicación : | 2011 |
DOI : | 10.1590/S0074-02762011000200021 |
URI : | http://ru.facmed.unam.mx/jspui/handle/FACMED_UNAM/A64 |
Aparece en las colecciones: | Artículos |
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