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http://ru.facmed.unam.mx/jspui/handle/FACMED_UNAM/A41
Título : | Maturation Phenotype of Peripheral Blood Monocyte/Macrophage After Stimulation with Lipopolysaccharides in Irritable Bowel Syndrome. |
Autor(es) : | Rodriguez Fandiño, Oscar Andres Hernandez Ruiz, Joselin Lopez Vidal, Yolanda Charúa, Luis Escobedo Gonzalez, Eustacio Galileo Schmulson Wasserman, Max Julio |
En : | Journal of Neurogastroenterology and Motility (1081-1710) vol. 23(2) 281-288 (2017) |
Número completo : | http://www.jnmjournal.org/journal/list.html?pn=vol&TG=vol&sm=&s_v=23&s_n=2&year=2017 |
Abstract : | BACKGROUND/AIMS: Abnormal immune regulation and increased intestinal permeability augmenting the passage of bacterial molecules that can activate immune cells, such as monocytes/macrophages, have been reported in irritable bowel syndrome (IBS). The aim was to compare the maturation phenotype of monocytes/macrophages (CD14+) from IBS patients and controls in the presence or absence of Escherichia coli lipopolysaccharides (LPS), in vitro. METHODS: Mononuclear cells were isolated from peripheral blood of 20 Rome II-IBS patients and 19 controls and cultured with or without LPS for 72 hours. The maturation phenotype was examined by flow cytometry as follows: M1-Early (CD11c+CD206-), M2-Advanced (CD11c-CD206+CX3CR1+); expression of membrane markers was reported as mean fluorescence intensity (MFI). The Mann-Whitney test was used and significance was set at P < 0.05. RESULTS: In CD14+ cells, CD11c expression decreased with vs without LPS both in IBS (MFI: 8766.0 ± 730.2 vs 12 920.0 ± 949.2, P < 0.001) and controls (8233.0 ± 613.9 vs 13 750.0 ± 743.3, P < 0.001). M1-Early cells without LPS, showed lower CD11c expression in IBS than controls (MFI: 11 540.0 ± 537.5 vs 13 860.0 ± 893.7, P = 0.040), while both groups showed less CD11c in response to LPS (P < 0.01). Furthermore, the percentage of "Intermediate" (CD11c+CD206+CX3CR1+) cells without LPS, was higher in IBS than controls (IBS = 9.5 ± 1.5% vs C = 4.9 ± 1.4%, P < 0.001). Finally, fractalkine receptor (CX3CR1) expression on M2-Advanced cells was increased when treated with LPS in controls but not in IBS (P < 0.001). CONCLUSIONS: The initial phase of monocyte/macrophage maturation appears to be more advanced in IBS compared to controls. However, the decreased CX3CR1 in patients with IBS, compared to controls, when stimulated with LPS suggests a state of immune activation in IBS. |
Palabras clave : | Lipopolisacáridos Monocitos Receptores de quimiocina CX3CR1 Síndrome de intestino irritable |
Fecha de publicación : | 2017 |
DOI : | https://doi.org/10.5056/jnm16137 |
URI : | http://ru.facmed.unam.mx/jspui/handle/FACMED_UNAM/A41 |
Aparece en las colecciones: | Artículos |
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