Use el DOI o este identificador para enlazar este recurso:
http://ru.facmed.unam.mx/jspui/handle/FACMED_UNAM/A79
Título : | Multiplex polymerase chain reaction as an improved method for screening Histoplasma capsulatum mating types. |
Autor(es) : | Almeida, Fernando Leonardo Barbedo Taylor Da Cunha E Mello, Maria Lucia Mauro de Medeiros Muniz Guimarães, Allan Jefferson Zancopé, Rosely |
En : | Memórias do Instituto Oswaldo Cruz (0074-0276) vol. 113(10), 1-4 (2018) |
Número completo : | https://memorias.ioc.fiocruz.br/content/past-issues/past/260 |
Abstract : | Histoplasmosis is a systemic mycosis infection caused by Histoplasma capsulatum, a heterothallic ascomycete. The sexual reproduction of this fungus is regulated by the mating type (MAT1) locus that contains MAT1-1 and MAT1-2 idiomorphs, which were identified by uniplex polymerase chain reaction (PCR). This study aimed to optimise single-step multiplex PCR for the accurate detection of the distinct mating types of H. capsulatum. Among the 26 isolates tested, 20 had MAT1-1 genotype, while six showed MAT1-2 genotype, in agreement with the uniplex PCR results. These results suggest that multiplex PCR is a fast and specific tool for screening H. capsulatum mating types. |
Palabras clave : | Micología Histoplasma capsulatum MAT1 Multiplex PCR |
Fecha de publicación : | 2018 |
DOI : | 10.1590/0074-02760180340 |
URI : | http://ru.facmed.unam.mx/jspui/handle/FACMED_UNAM/A79 |
Aparece en las colecciones: | Artículos |
Texto completo:
Archivo | Descripción | Tamaño | Formato | |
---|---|---|---|---|
FACMED_UNAMA79.pdf | 406.45 kB | Adobe PDF | Visualizar/Abrir |
Este recurso está sujeto a una Licencia Creative Commons