Células flama de Taenia solium

Abstract

The worm was obtained from the small bowel of a golden hamster under 30 days of immunosuppression previously infected orally with 10 Taenia solium metacestodes. The tissue was included in resin and 10 micron thick sections were stained with fluoresceinated antibodies directed against alpha-tubulin (green) and actin (red). The slices were observed under an OLYMPUS confocal microscope, at 60x magnification with a digital zoom of 10. The distribution of alpha-tubulin and actin in the flame cells is observed while the nuclei were stained with DAPI (blue) (A and B) and "Nomarsky" technique by Interdifferential contrast optics where used (B and C). A) There are numerous cilia marked with alpha-tubulin and the structure that surrounds the cilia in red is due to the presence of actin; B) Corresponds to the combination of the images of flame cells stained with fluoresceinated antibodies and Nomarsky; C) Flame cells under Nomarsky.