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http://ru.facmed.unam.mx/jspui/handle/FACMED_UNAM/A109
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Vega Angeles, Vera Teresa | |
dc.contributor.author | Terrazas Valdes, Luis Ignacio | |
dc.contributor.author | Ledesma Soto, Yadira | |
dc.contributor.author | Lucía Jiménez | |
dc.contributor.author | Landa Piedra, Abraham | |
dc.coverage.spatial | NL | |
dc.date.accessioned | 2019-06-17T17:23:07Z | - |
dc.date.available | 2019-06-17T17:23:07Z | - |
dc.date.issued | 2019 | |
dc.identifier.uri | http://ru.facmed.unam.mx/jspui/handle/FACMED_UNAM/A109 | - |
dc.description.abstract | Glutathione (GSH) transferase (GST) is an essential enzyme in cestodes for the detoxification of xenobiotics. In Taenia solium, two GSTs (Ts25GST and Ts26GST kDa) were isolated as a fraction (SGSTF) by GSH-Sepharose-4B. Both are located on the tegument. Immunization assays with SGSTF reduced up to 90% of the parasitic load in a murine model of cysticercosis. It prompted us to investigate how SGSTF induces this protective immune response. To test it, we exposed peritoneal macrophages to SGSTF for 24 h; such exposure favored the production of IL-12, TNF, and IL-10 as well as the expression of nitric oxide synthase 2 inducible (Nos2) and CD86, but did not induce the expression of chitinase-like 3 (Chil3). Confocal microscopy showed that the macrophages internalize the SGSTF which co-localized after 1 h with MHC-II in their plasma membranes. Macrophages exposed to SGSTF and co-cultured with anti-CD3 pre-activated T CD4+ cells, enhanced the proliferation of CD4+ cells, induced high interferon-? (IFN-?) secretion, and elevated the expression of CD25 and CD69, molecules associated with cell activation. Similar assay using T CD4+ cells from DO11.10 mice and ovalbumin (OVA) peptide+SGSTF as stimuli, showed enhanced cell proliferation and OVA-specific IFN-? secretion. These data are in-line with those indicating that the P1, P5, and P6 peptides of Schistosoma japonicum 28GST highly promote T-cell proliferation and Th1 response in vitro We found that such peptides are also present on Ts25GST and Ts26GST. It suggests that SGSTF activates peritoneal macrophages to a classically activated-like phenotype, and that these macrophages induce the differentiation of T CD4+ cells toward a Th1-type response. | |
dc.language.iso | en | |
dc.publisher | Kluwer Academic Publishers | |
dc.rights | openAccess | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0 | |
dc.subject | PArasitología | |
dc.subject | Macrófagos clásicamente activados | |
dc.subject | Glutación transferasa | |
dc.subject | Taenia | |
dc.subject | Respuesta inmune Th1 | |
dc.subject | Cisticercosis | |
dc.subject.classification | Biología y Química | |
dc.subject.other | Parasitology | |
dc.subject.other | Classically activated macrophages | |
dc.subject.other | Glutathione transferase | |
dc.subject.other | Taenia | |
dc.subject.other | Th1-immune response | |
dc.subject.other | Cysticercosis | |
dc.title | Taenia solium glutathione transferase fraction activates macrophages and favors the development of Th1-type response. | |
dc.type | Artículo | |
dc.type | publishedVersion | |
dcterms.bibliographicCitation | Bioscience Reports (0144-8463) vol. 39(1), 1-16 (2019) | |
dcterms.creator | Vega Angeles, Vera Teresa::cvu::378618 | |
dcterms.creator | Terrazas Valdes, Luis Ignacio::cvu::13398 | |
dcterms.creator | Ledesma Soto, Yadira::cvu::205366 | |
dcterms.creator | Lucía Jiménez::orcid::0000-0001-9555-8071 | |
dcterms.creator | Landa Piedra, Abraham::cvu::9301 | |
dc.identifier.doi | 10.1042/BSR20181132 | |
dc.relation.ispartofjournal | http://www.bioscirep.org/content/39/1 | |
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